Clentiazem reduces infarct size in rabbit middle cerebral artery occlusion.

نویسندگان

  • L Kaminow
  • J Bevan
چکیده

We assessed the value of pretreatment with clentiazem (8-chlorodiltiazem), a diltiazem derivative with cerebroselective properties, on the consequences of surgical occlusion of the middle cerebral artery via a transorbital approach in 38 rabbits. Nineteen rabbits received 1.7 (n = 5), 5 (n = 8), or 15 (n = 6) mg/kg clentiazem orally four times a day for 24 hours before and 48 hours after occlusion. Upon sacrifice, a segment of the right middle cerebral artery distal to the occlusion and a corresponding segment from the nonoccluded left middle cerebral artery were mounted on myographs for in vitro study of their reactivity to histamine, acetylcholine, serotonin, norepinephrine, and electrical stimulation of intramural sympathetic nerves. Morphometric measurements of 2,3,5-triphenyltetrazolium chloride-stained brain slices permitted us to estimate infarct volume. Pretreatment with 1.7, 5, and 15 mg/kg clentiazem significantly reduced infarct volume (p less than 0.05, p less than 0.01, and p less than 0.01, respectively). Mean infarct volume of the 15 mg/kg-treated group was only 4% that of the untreated group. There were no postoperative deaths in any treated group compared with a death rate of 36% in the untreated group. Mean values for vascular smooth muscle contractility to histamine and relaxation to acetylcholine were significantly enhanced in vessels from treated rabbits. These studies indicate that pretreatment with clentiazem offers cerebral protection and significantly reduces infarct volume as well as arterial wall damage beyond the occlusion.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Investigate the effect of TRPV1 receptor antagonist (AMG9810) on stroke outcome in the permanent middle cerebral artery occlusion in male rat

Introduction: TRPV1 is a non-selective cation channel with high permeability to calcium ions, and is also involved in the development of neurogenic and inflammatory pain. The increase in intracellular calcium plays a role in worsening of stroke. In the present study we investigated the effect of (AMG9810) TRPV1 receptor antagonist on stroke outcome in the permanent middle cerebral artery occlus...

متن کامل

بررسی اثر انسداد گذرای شریان مرکزی در کاهش آسیب‌های مغزی در مدل سکته‌ی مغزی رت

Background and Objective: Recent studies suggest that sub-lethal ischemia protect the brain from subsequent ischemic injuries. This study was an effort to identify and shed light on the nature of changes in the blood brain barrier permeability and brain edema. Materials and Methods: Rats were divided into four main experimental groups, each of 21 animals. The first group acted as a model of isc...

متن کامل

Inhibition of nitric oxide synthase activity improves focal cerebral damage induced by cerebral ischemia/reperfusion in normotensive rats

Introduction: Nitric oxide seems to play a dual role in ischemia/reperfusion injury. Few studies have investigated whether it exacerbates or improves brain edema. In the present study, we inhibited the activity of nitric oxide synthase by L-NAME and evaluated the cerebral infarct volume, tissue swelling and brain edema, alongside the measurement of blood flow of the ischemic region. Methods...

متن کامل

Attenuating of NF-Κb/VCAM-1 Expression in Middle Cerebral Artery Occlusion (MCAO) Model by Viola Odorata: Protection Against Injury Ischemia- Reperfusion Injury in Rats

Background: The death of neurons and cerebral edema are the main consequences of stroke. However, inflammatory processes play a key role in aggravating cerebral damage following stroke. The aim of this study was to investigate the effects of Viola odorant extract (VOE) on infarct volume (IV), neurologic deficits (ND), and expression of NF-κB and VCAM-1 in the MCAO model. Method: The animals we...

متن کامل

Trandolapril reduces infarction area after middle cerebral artery occlusion in rats.

In this study, we investigated whether angiotensin-converting enzyme (ACE) is involved in the progression of cerebral infarct lesions after middle cerebral artery (MCA) occlusion in rats. After placebo or trandolapril was administered orally for 7 days, we infarcted in the territory of the right MCA by extracranial vascular occlusion and studied the effect of trandolapril on brain ACE activity ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Stroke

دوره 22 2  شماره 

صفحات  -

تاریخ انتشار 1991